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N-Glycosylation and Plant Cell Growth

  • Christiane Veit
  • Ulrike Vavra
  • Richard StrasserEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1242)

Abstract

N-linked glycosylation is one of the most prevalent cotranslational protein modifications in plants. It is initiated by a conserved process in the endoplasmic reticulum and subsequently involves a series of different N-glycan maturation steps that take place in the ER and Golgi apparatus. Despite our vast knowledge on the different processing steps we still understand very little about the role of characteristic glycoforms present on individual plant glycoproteins. Here, we describe convenient tools that allow the fast and reliable characterization of N-glycosylation on plant glycoproteins. The presented protocols can be adopted to other plant species and to the characterization of N-glycans from different glycoproteins.

Key words

N-glycosylation Glycoprotein Oligomannosidic N-glycans Complex N-glycans Immunoblot Endoglycosidase Underglycosylation Endoplasmic reticulum Golgi Arabidopsis thaliana 

Notes

Acknowledgements

This work was supported by a grant from the Austrian Science Fund (FWF): P23906.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Christiane Veit
    • 1
  • Ulrike Vavra
    • 1
  • Richard Strasser
    • 1
    Email author
  1. 1.Department of Applied Genetics and Cell BiologyUniversity of Natural Resources and Life SciencesViennaAustria

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