N-Glycosylation and Plant Cell Growth
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N-linked glycosylation is one of the most prevalent cotranslational protein modifications in plants. It is initiated by a conserved process in the endoplasmic reticulum and subsequently involves a series of different N-glycan maturation steps that take place in the ER and Golgi apparatus. Despite our vast knowledge on the different processing steps we still understand very little about the role of characteristic glycoforms present on individual plant glycoproteins. Here, we describe convenient tools that allow the fast and reliable characterization of N-glycosylation on plant glycoproteins. The presented protocols can be adopted to other plant species and to the characterization of N-glycans from different glycoproteins.
Key wordsN-glycosylation Glycoprotein Oligomannosidic N-glycans Complex N-glycans Immunoblot Endoglycosidase Underglycosylation Endoplasmic reticulum Golgi Arabidopsis thaliana
This work was supported by a grant from the Austrian Science Fund (FWF): P23906.