Live Cell Imaging of the Cytoskeleton and Cell Wall Enzymes in Plant Cells
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The use of live imaging techniques to visualize the dynamic changes and interactions within plant cells has given us detailed information on the function and organization of the cytoskeleton and cell wall associated proteins. This information has grown with the constant improvement in imaging hardware and molecular tools. In this chapter, we describe the procedure for the preparation and live visualization of fluorescent protein fusions associated with the cytoskeleton and the cell wall in Arabidopsis.
Key wordsLive cell imaging Microtubules Actin filaments Cell wall Point scanning confocal microscopy Spinning disc microscopy Hypocotyls Xylem vessels
We would like to thank Dr. Staffan Persson at the Max Planck Institute of Molecular Plant Physiology for his comments on the chapter.