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Quantification of Fluorescent Reporters in Plant Cells

  • Michael Pound
  • Andrew P. French
  • Darren M. WellsEmail author
Protocol
  • 3.4k Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 1242)

Abstract

Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification of fluorescence intensity in plant cells from confocal laser scanning microscope images using semiautomated software and image analysis techniques.

Key words

Fluorescent reporters Image analysis CellSeT DII-VENUS 

Notes

Acknowledgments

The authors wish to acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC) and Engineering and Physical Sciences Research Council (EPSRC) funding to the Centre for Plant Integrative Biology (CPIB) and BBSRC responsive mode grant support to M.P.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Michael Pound
    • 1
  • Andrew P. French
    • 1
  • Darren M. Wells
    • 1
    Email author
  1. 1.Centre for Plant Integrative Biology, School of BiosciencesUniversity of NottinghamSutton BoningtonUK

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