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Modification Interference Analysis of the Ribosome

  • Simpson JosephEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1240)

Abstract

RNAs are versatile molecules involved in myriad functions in the cell. To understand how a RNA molecule functions in the cell it is important to identify the nucleotides in the RNA molecule that are important for its structure and function. There are several biochemical methods such as footprinting, cross-linking, and modification interference analysis that can be used to study RNA–RNA and RNA–protein interactions. Ribosome is a classical example of a RNA–protein complex that has been extensively studied using these methods. Here, we describe a modification interference method that was used to identify bases in 16S rRNA that are important for the translocation of the mRNA–tRNA complex by the ribosome.

Key words

Ribosome mRNA tRNA EF-G Translocation Chemical modification rRNA Cross-linking Footprinting 

Notes

Acknowledgement

This work was supported by a grant from the National Institutes of Health (GM65265).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of California at San DiegoLa JollaUSA

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