Modification Interference Analysis of the Ribosome
RNAs are versatile molecules involved in myriad functions in the cell. To understand how a RNA molecule functions in the cell it is important to identify the nucleotides in the RNA molecule that are important for its structure and function. There are several biochemical methods such as footprinting, cross-linking, and modification interference analysis that can be used to study RNA–RNA and RNA–protein interactions. Ribosome is a classical example of a RNA–protein complex that has been extensively studied using these methods. Here, we describe a modification interference method that was used to identify bases in 16S rRNA that are important for the translocation of the mRNA–tRNA complex by the ribosome.
Key wordsRibosome mRNA tRNA EF-G Translocation Chemical modification rRNA Cross-linking Footprinting
This work was supported by a grant from the National Institutes of Health (GM65265).
- 15.Merryman C, Noller HF (1998) Footprinting and modification-interference analysis of binding sites on RNA. In: Smith CWJ (ed) RNA: protein interaction. Oxford University Press, New York, pp 237–253Google Scholar