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RNA–RNA SELEX

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1240)

Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA). Here, we describe the separation of the RNA aptamers binding to an RNA stem-loop target with affinity chromatography using the column attached the target RNA and nondenaturing polyacrylamide gel electrophoresis to obtain a single predominant RNA aptamer family.

Key words

Systematic evolution of ligands by exponential enrichment (SELEX) RNA aptamer Affinity chromatography Electrophoretic mobility shift assay (EMSA) NaIO4 Nondenaturing polyacrylamide gel electrophoresis 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Applied ChemistryCheongju UniversityCheongju ChungbukS. Korea

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