RNA-Directed Recombination of RNA In Vitro
Construction of long RNAs can be achieved in vitro by using ribozymes to recombine shorter RNAs. This can be a useful technique to prepare RNAs when the final product is either very long or contains chemical modifications that are difficult to incorporate using standard in vitro transcription techniques. Here, we describe the use of the Azoarcus group I intron ribozyme to recombine shorter RNAs into longer ones. This ribozyme is a generalized RNA recombinase ribozyme that operates rapidly and with high efficiency.
Key wordsElectrophoresis Polymerase chain reaction Recombination Ribozyme Group I intron Azoarcus ribozyme Transcription
We would like to thank A. Burton and B. Larson for help during the preparation of this manuscript. This work was supported by the NASA grant NNX10AR15G to N.L.
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