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RNA-Directed Recombination of RNA In Vitro

  • Niles Lehman
  • Nilesh Vaidya
  • Jessica A. M. Yeates
Part of the Methods in Molecular Biology book series (MIMB, volume 1240)

Abstract

Construction of long RNAs can be achieved in vitro by using ribozymes to recombine shorter RNAs. This can be a useful technique to prepare RNAs when the final product is either very long or contains chemical modifications that are difficult to incorporate using standard in vitro transcription techniques. Here, we describe the use of the Azoarcus group I intron ribozyme to recombine shorter RNAs into longer ones. This ribozyme is a generalized RNA recombinase ribozyme that operates rapidly and with high efficiency.

Key words

Electrophoresis Polymerase chain reaction Recombination Ribozyme Group I intron Azoarcus ribozyme Transcription 

Notes

Acknowledgements

We would like to thank A. Burton and B. Larson for help during the preparation of this manuscript. This work was supported by the NASA grant NNX10AR15G to N.L.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Niles Lehman
    • 1
  • Nilesh Vaidya
    • 2
  • Jessica A. M. Yeates
    • 1
  1. 1.Department of ChemistryPortland State UniversityPortlandUSA
  2. 2.Department of Chemical and Biological EngineeringPrinceton UniversityPrincetonUSA

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