Electrophoretic Mobility Shift Assays: Analysis of tRNA Binding to the T Box Riboswitch Antiterminator RNA

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1240)

Abstract

Changes in electrophoretic mobility upon complex formation with RNA can be used to probe structure–function relationships that are critical for complex formation. Here, we describe the application of this technique to monitor tRNA binding to the T box riboswitch antiterminator RNA.

Key words

EMSA Gel mobility shift T box riboswitch RNA Antiterminator Drug discovery 

Notes

Acknowledgement

We thank the National Institutes of Health (GM61048, GM073188) for support of this work. We also thank Ohio University for support of the BioMolecular Innovation and Technology (BMIT) project.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryOhio UniversityAthensUSA

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