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Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains

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Chromosomal Mutagenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1239))

Abstract

Freeze-drying spermatozoa is the ultimate method for the maintenance of animal strains, in that the gametes can be preserved for a long time in a refrigerator at 4 °C. Furthermore, it is possible to realize easy and safe transportation of spermatozoa at an ambient temperature that requires neither liquid nitrogen nor dry ice. Freeze-drying spermatozoa has been established as a new method for storing genetic resources instead of cryopreservation using liquid nitrogen. This chapter introduces our latest protocols for freeze-drying of mouse and rat spermatozoa, and the anticipated results of the fertilizing ability of these gametes following long-term preservation or transportation.

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Author information

Authors and Affiliations

  1. Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan

    Takehito Kaneko

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  1. Takehito Kaneko
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Correspondence to Takehito Kaneko .

Editor information

Editors and Affiliations

  1. Department of Genetics, Genome Engineering and iPSC Center, Washington University School of Medicine, St. Louis, Missouri, USA

    Shondra M. Pruett-Miller

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Kaneko, T. (2015). Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains. In: Pruett-Miller, S. (eds) Chromosomal Mutagenesis. Methods in Molecular Biology, vol 1239. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1862-1_19

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  • DOI: https://doi.org/10.1007/978-1-4939-1862-1_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1861-4

  • Online ISBN: 978-1-4939-1862-1

  • eBook Packages: Springer Protocols

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