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Stem Cell Protocols pp 231–241Cite as

Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1235))

Abstract

Clonal culture of stem cells is crucial for their identification, and the characterization of the cellular and molecular mechanisms that regulate their proliferation and differentiation. In the adult mouse lung, epithelial stem/progenitor cells are defined by the phenotype CD45neg CD31neg EpCAMpos CD104pos CD24low. Here we describe a tissue dissociation and flow cytometry strategy for the detection and isolation of adult mouse lung epithelial stem/progenitor cells, and a three-dimensional colony-forming assay for their clonal culture in vitro.

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Acknowledgements

This work was supported by grants from the Australian National Health and Medical Research Council (NHMRC) and the Australian Stem Cell Centre.

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Authors and Affiliations

  1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Grattan Street, Parkville, VIC, 3010, Australia

    Jonathan L. McQualter & Ivan Bertoncello

Authors
  1. Jonathan L. McQualter
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  2. Ivan Bertoncello
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Corresponding author

Correspondence to Jonathan L. McQualter .

Editor information

Editors and Affiliations

  1. HemoGenix, Inc., Colorado Springs, Colorado, USA

    Ivan N. Rich

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McQualter, J.L., Bertoncello, I. (2015). Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor Cells. In: Rich, I. (eds) Stem Cell Protocols. Methods in Molecular Biology, vol 1235. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1785-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1785-3_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1784-6

  • Online ISBN: 978-1-4939-1785-3

  • eBook Packages: Springer Protocols

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