Abstract
Identification of G protein-coupled receptor (GPCR)-interacting proteins is an intense subject of current research. However, confirmation and characterization of identified interactions can be difficult with GPCRs, especially at the endogenous level. Here, we describe how we characterized the interaction between the prostaglandin D2 DP1 receptor and the intracellular l-type prostaglandin D synthase by in vitro pull-down assays using purified recombinant GST- and His-tagged proteins, by co-immunoprecipitation of overexpressed Flag- and HA-tagged proteins, and by co-immunoprecipitation of endogenous proteins.
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Acknowledgements
This work was supported by a grant from the Canadian Institutes of Health Research and by the André-Lussier Research Chair to J.L.P.
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Binda, C., Parent, JL. (2015). Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular l-Prostaglandin D Synthase. In: Allen, B., Hébert, T. (eds) Nuclear G-Protein Coupled Receptors. Methods in Molecular Biology, vol 1234. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1755-6_6
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DOI: https://doi.org/10.1007/978-1-4939-1755-6_6
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