Abstract
The measurement of changes in the transcriptome is a common end point for various pathologic and pharmacologic studies. In recent years, with the discovery of a host of potential pharmacologic targets located directly on the nuclear membrane, the need to assess their potential control over the transcriptome has arisen. Here we present techniques for assessing changes in gene expression in isolated nuclei in response to stimulation by endogenous GPCRs on the nuclear membrane.
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Acknowledgements
This work was supported by grants from the Canadian Institutes of Health Research (MOP-64183 and MOP-125970 to BGA and MOP-79354 and MOP-119530 to TEH) and the Fondation des Maladies du Coeur du Québec (to BA).
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Vaniotis, G., Gora, S., Nantel, A., Hébert, T.E., Allen, B.G. (2015). Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei. In: Allen, B., Hébert, T. (eds) Nuclear G-Protein Coupled Receptors. Methods in Molecular Biology, vol 1234. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1755-6_15
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DOI: https://doi.org/10.1007/978-1-4939-1755-6_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1754-9
Online ISBN: 978-1-4939-1755-6
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