The Illumina-Solexa Sequencing Protocol for Bacterial Genomes

  • Zhenfei Hu
  • Lei Cheng
  • Hai WangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1231)


Based on reversible dye-terminators technology, the Illumina-solexa sequencing platform enables rapid sequencing-by-synthesis (SBS) of large DNA stretches spanning entire genomes, with the latest instruments capable of producing hundreds of gigabases of data in a single sequencing run. Illumina’s NGS instruments powerfully combine the flexibility of single reads with short- and long-insert paired-end reads, and enable a wide range of DNA sequencing applications. Here, we describe the paired-end library preparation with an average insert size of 470 bp, 2 kbp, and 6 kbp, together with the DNA cluster generation and sequencing procedure of E. coli O104:H4 genome on Illumina Hiseq 2000 platform.

Key words

Illumina Solexa Hiseq Reversible dye-terminators Sequencing by synthesis Single reads Paired-end reads Library preparation Cluster generation 



This work was supported by E. coli O104:H4 Genome Analysis Crowd-Sourcing Consortium.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.BGIShenzenChina

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