The Pyrosequencing Protocol for Bacterial Genomes

  • Ermanno RizziEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1231)


The pyrosequencing methodology was applied in 2005 by 454 Lifesciences to the emerging field of next generation sequencing (NGS), revolutionizing the way of DNA sequencing. In the last years the same strategy grew up and was technologically updated, reaching a high throughput in terms of amount of generated sequences (reads) per run and in terms of length of sequence up to values of 800–1,000 bases. These features of pyrosequencing perfectly fit to bacterial genome sequencing for the de novo assemblies and resequencing as well. The approaches of shotgun and paired ends sequencing allow the bacterial genome finishing providing a high-quality data in few days with unprecedented results.

Key words

NGS Pyrosequencing Reads Bacterial genomes Paired ends 



The author acknowledges the two “FIRB-Futuro in Ricerca” grants from the Italian Minister of Education, Universities and Research (MIUR): RBFR08U07M and RBFR126B8I.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.National Research Council (CNR)Institute for Biomedical Technologies (ITB)SegrateItaly

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