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Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration

  • Hong Qiu
  • Wenyuan Xiao
  • Jingwen Yue
  • Lianchun WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)

Abstract

Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.

Key words

Heparan sulfate Endothelial cell Migration Slit3 Robo4 Angiogenesis 

Notes

Acknowledgement

This work was supported by grants from NIH R01HL093339 (L.W.) and RR005351/GM103390 (L.W.).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Hong Qiu
    • 1
  • Wenyuan Xiao
    • 1
  • Jingwen Yue
    • 1
  • Lianchun Wang
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research CenterUniversity of GeorgiaAthensUSA

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