Chemical Modification of Heparin and Heparosan

  • Karthik Raman
  • Balagurunathan Kuberan
  • Sailaja ArungundramEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)


Heparin is a potent clinically used anticoagulant. It is a heterogeneous mixture of polymers that contain a variety of sulfation patterns. However, only 3-O sulfonated heparin pentasaccharide units have been proven to bind to antithrombin and elicit an anticoagulant response. Heparins with other sulfation patterns are able to bind to a variety of other proteins such as FGF, VEGF, and CXCL-3. By modulating heparin’s sulfation pattern, it is possible to generate polymers that can regulate biological processes beyond hemostasis. Here we describe a variety of simple chemical modification methods, N-acetylation, N-deacetylation, N-sulfation, O-sulfation, 2-O desulfation, and complete desulfation, to prepare heparin-like polymers with distinct sulfation patterns.

Key words

Heparin Desulfation O-sulfation Deacetylation N-sulfation 



This work was supported in part by NIH grants (P01HL107152 and R01GM075168) to B.K. and by the NIH fellowship F31CA168198 to K.R.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Karthik Raman
    • 2
  • Balagurunathan Kuberan
    • 1
    • 2
    • 3
  • Sailaja Arungundram
    • 4
    Email author
  1. 1.Department of Medicinal ChemistryUniversity of UtahSalt Lake CityUSA
  2. 2.Department of BioengineeringUniversity of UtahSalt Lake CityUSA
  3. 3.Interdepartmental Program in NeuroscienceUniversity of UtahSalt Lake CityUSA
  4. 4.Academic ProgramsSouth Seattle CollegeSeattleUSA

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