Genetic Approaches in the Study of Heparan Sulfate Functions in Drosophila
Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).
Key wordsDrosophila melanogaster HSPG GAL4/UAS TARGET MARCM
We are grateful to Daniel Levings and Pui Choi for helpful comments to the manuscript. The authors are supported by Japan Society for the Promotion of Science (to M. T.) and National Institutes of Health Grant R01 HD042769 (to H. N.).
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