The intrinsic and extrinsic factors that contribute to stem and neuronal precursor cell maintenance and/or differentiation remain poorly understood. Proteoglycans, major residents of the stem cell microenvironment, modulate key signaling cues and are of particular importance. We have taken a loss-of-function approach, by developing a library of bacterial lyases and sulfatases to specifically remodel the ECM and test the functional role of glycosaminoglycans (GAGs) in cell self-renewal, maintenance, and differentiation.
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
This work was supported by NIH grants HD-009402, HD 017332, HD 054275, HD 06976, and 10035 from the Mizutani Foundation for Glycoscience. We thank James Mensch for help in assembling and proofing this manuscript.
Johnson CE, Crawford BE et al (2007) Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to sox1-enhanced green fluorescent protein-expressing neural progenitor cells. Stem Cells 25(8):1913–1923PubMedCrossRefGoogle Scholar
Sirko S, von Holst A et al (2007) Chondroitin sulfate glycosaminoglycans control proliferation, radial glia cell differentiation and neurogenesis in neural stem/progenitor cells. Development 134(15):2727–2738PubMedCrossRefGoogle Scholar
Cortes M, Baria AT et al (2009) Sulfation of chondroitin sulfate proteoglycans is necessary for proper Indian hedgehog signaling in the developing growth plate. Development 136(10):1697–1706PubMedCentralPubMedCrossRefGoogle Scholar
Domowicz M, Mangoura D et al (2000) Cell specific-chondroitin sulfate proteoglycan expression during CNS morphogenesis in the chick embryo. Int J Dev Neurosci 18(7):629–641PubMedCrossRefGoogle Scholar
Calabro A, Benavides M et al (2000) Microanalysis of enzyme digests of hyaluronan and chondroitin/dermatan sulfate by fluorophore-assisted carbohydrate electrophoresis (FACE). Glycobiology 10(3):273–281PubMedCrossRefGoogle Scholar
Calabro A, Hascall VC et al (2000) Adaptation of FACE methodology for microanalysis of total hyaluronan and chondroitin sulfate composition from cartilage. Glycobiology 10(3):283–293PubMedCrossRefGoogle Scholar
Calabro A, Midura R et al (2001) Fluorophore-assisted carbohydrate electrophoresis (FACE) of glycosaminoglycans. Osteoarthritis Cartilage 9(Suppl A):S16–S22PubMedCrossRefGoogle Scholar