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Mapping Proteoglycan Functions with Glycosidases

  • Mauricio Cortes
  • Leslie K. Cortes
  • Nancy B. SchwartzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)

Abstract

The intrinsic and extrinsic factors that contribute to stem and neuronal precursor cell maintenance and/or differentiation remain poorly understood. Proteoglycans, major residents of the stem cell microenvironment, modulate key signaling cues and are of particular importance. We have taken a loss-of-function approach, by developing a library of bacterial lyases and sulfatases to specifically remodel the ECM and test the functional role of glycosaminoglycans (GAGs) in cell self-renewal, maintenance, and differentiation.

Key words

Proteoglycan Glycosaminoglycan Chondroitin sulfate Heparan sulfate Glycosidase ES cells Cloning Cortical slices 

Notes

Acknowledgements

This work was supported by NIH grants HD-009402, HD 017332, HD 054275, HD 06976, and 10035 from the Mizutani Foundation for Glycoscience. We thank James Mensch for help in assembling and proofing this manuscript.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Mauricio Cortes
    • 1
    • 2
  • Leslie K. Cortes
    • 1
    • 3
  • Nancy B. Schwartz
    • 1
    Email author
  1. 1.Department of PediatricsUniversity of ChicagoChicagoUSA
  2. 2.Department of Pathology, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA
  3. 3.New England BiolabsIpswichUSA

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