Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)

Abstract

In situ hybridization provides information for understanding the localization of gene expression in various tissues. The relative expression levels of mRNAs in a single cell can be sensitively visualized by this technique. Furthermore, since in situ hybridization is a histological technique, tissue structure is maintained after fixation, and it is possible to accurately identify cell types. We have examined the expression of heparan sulfate sulfotransferases by in situ hybridization to better understand the functions of heparan sulfate in the development of mouse nervous system. This chapter describes methods of in situ hybridization analyses using cRNA probes labeled with nonradioactive nucleotides.

Key words

In situ hybridization cRNA probe Digoxigenin-11-UTP Paraffin-embedded section Heparan sulfate 3-O-sulfotransferase 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Faculty of Applied Biological Sciences, Department of Applied Life ScienceGifu UniversityGifuJapan
  2. 2.Tokyo Metropolitan Institute of Medical ScienceSetagaya-kuJapan

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