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Analysis of Hyaluronan Synthase Activity

  • Davide Vigetti
  • Evgenia Karousou
  • Manuela Viola
  • Alberto PassiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)

Abstract

Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several functions (i.e., cell proliferation and migration, inflammation), its presence in the tissues can have positive or negative effects. HA synthases (HAS) are a family of three isoenzymes located on the plasma membrane that are responsible for the production of such polysaccharide and, therefore, their activity is critical to determine the accumulation of HA in tissues. Here, we describe a nonradioactive method to quantify the HAS enzymatic activity in crude cellular membrane preparation.

Key words

Enzymatic activity UDP sugars Glycosaminoglycan synthesis Hyaluronan Hyaluronan synthase 

Notes

Acknowledgements

The authors acknowledge the “Centro Grandi Attrezzature per la Ricerca Biomedica”, Università degli Studi dell’Insubria, for instruments availability and the PhD School in Biological and Medical Sciences.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Davide Vigetti
    • 1
  • Evgenia Karousou
    • 1
  • Manuela Viola
    • 1
  • Alberto Passi
    • 1
    Email author
  1. 1.Dipartimento di Scienze Chirurgiche e MorfologicheUniversità degli Studi dell’InsubriaVareseItaly

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