Heparan Sulfate Structure: Methods to Study N-Sulfation and NDST Action

  • Anders Dagälv
  • Anders Lundequist
  • Beata Filipek-Górniok
  • Tabea Dierker
  • Inger Eriksson
  • Lena KjellénEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)


Heparan sulfate proteoglycans are important modulators of cellular processes where the negatively charged polysaccharide chains interact with target proteins. The sulfation pattern of the heparan sulfate chains will determine the proteins that will bind and the affinity of the interactions. The N-deacetylase/N-sulfotransferase (NDST) enzymes are of key importance during heparan sulfate biosynthesis when the sulfation pattern is determined. In this chapter, metabolic labeling of heparan sulfate with [35S]sulfate or [3H]glucosamine in cell cultures is described, in addition to characterization of polysaccharide chain length and degree of N-sulfation. Methods to measure NDST enzyme activity are also presented.

Key words

Heparan sulfate biosynthesis N-deacetylase/N-sulfotransferase N-sulfation ELISA JM-403 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Anders Dagälv
    • 1
  • Anders Lundequist
    • 1
  • Beata Filipek-Górniok
    • 1
  • Tabea Dierker
    • 1
  • Inger Eriksson
    • 1
  • Lena Kjellén
    • 1
    Email author
  1. 1.Department of Medical Biochemistry and Microbiology, Science for Life LaboratoryUppsala UniversityUppsalaSweden

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