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Validated Capillary Electrophoretic Assays for Disaccharide Composition Analysis of Galactosaminoglycans in Biologic Samples and Drugs/Nutraceuticals

  • Athanasia P. Asimakopoulou
  • Christina Malavaki
  • Nikolaos A. Afratis
  • Achilleas D. Theocharis
  • Fotini N. Lamari
  • Nikos K. KaramanosEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)

Abstract

Capillary electrophoresis is a separation technique with high resolving power and sensitivity with applications in glycosaminoglycan analysis. In this chapter, we present validated protocols for determining the variously sulfated chondroitin or dermatan sulfate-derived disaccharides. These approaches involve degradation of the polysaccharides with specific chondro/dermato-lyases and electrophoretic analysis with capillary zone electrophoresis in a low pH operating buffer and reversed polarity. This methodology has been applied to drug/nutraceutical formulations or to biologic samples (blood serum, lens capsule) and has been validated. Analysis of biologic tissue samples is often more demanding in terms of detection sensitivity, and thus concentration pretreatment steps and/or a derivatization step with 2-aminoacridone are often advisable.

Key words

Disaccharides Capillary electrophoresis 2-Aminoacridone Enzymic treatment Glycosaminoglycans Validation 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Athanasia P. Asimakopoulou
    • 1
  • Christina Malavaki
    • 1
  • Nikolaos A. Afratis
    • 1
  • Achilleas D. Theocharis
    • 1
  • Fotini N. Lamari
    • 2
  • Nikos K. Karamanos
    • 1
    Email author
  1. 1.Laboratory of Biochemistry, Department of ChemistryUniversity of PatrasPatrasGreece
  2. 2.Department of PharmacyUniversity of PatrasPatrasGreece

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