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Chromatographic Molecular Weight Measurements for Heparin, Its Fragments and Fractions, and Other Glycosaminoglycans

  • Barbara MulloyEmail author
  • John Hogwood
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1229)

Abstract

Glycosaminoglycan samples are usually polydisperse, consisting of molecules with differing length and differing sequence. Methods for measuring the molecular weight of heparin have been developed to assure the quality and consistency of heparin products for medicinal use, and these methods can be applied in other laboratory contexts. In the method described here, high-performance gel permeation chromatography is calibrated using appropriate heparin molecular weight markers or a single broad standard calibrant, and used to characterize the molecular weight distribution of polydisperse samples or the peak molecular weight of monodisperse, or approximately monodisperse, heparin fractions. The same technology can be adapted for use with other glycosaminoglycans.

Key words

Gel permeation chromatography Heparin Heparan sulfate Molecular weight 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.National Institute for Biological Standards and ControlHertfordshireUK
  2. 2.Institute of Pharmaceutical ScienceKing’s College LondonLondonUK

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