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Opioid Receptors: Methods for Detection and Their Modes of Actions in the Eye

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Opioid Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1230))

Abstract

This study provides evidence for the presence of opioid-receptors in the retina, optic nerve, and optic nerve head astrocytes. These receptors were measured by more than one technique including Western blotting, immunohistochemistry, and functional assays such as scotopic electroretinogram (ERG) and Pattern ERG. I also have provided evidence in recently published work from my laboratory that opioid receptors, more specifically δ-opioid receptors, play crucial roles in retina neuroprotection against ischemic and glaucomatous injuries. This chapter provides detailed procedures to measure opioid receptor activation and their roles in retina neuroprotection using functional assays such as scotopic ERG and pattern ERG.

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Acknowledgements

Supported in part by NIH/NEI grant EY019081 and an unrestricted grant to MUSC-SEI from Research to Prevent Blindness, New York, NY.

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Authors and Affiliations

  1. Department of Ophthalmology, Hewitt Laboratory of the Ola B. Williams Glaucoma Center, Storm Eye Institute, Medical University of South Carolina, Room 713, 167 Ashley Avenue, Charleston, SC, 29425, USA

    Shahid Husain Ph.D.

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  1. Shahid Husain Ph.D.
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Corresponding author

Correspondence to Shahid Husain Ph.D. .

Editor information

Editors and Affiliations

  1. University of Bologna, Bologna, Italy

    Santi M. Spampinato

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Husain, S. (2015). Opioid Receptors: Methods for Detection and Their Modes of Actions in the Eye. In: Spampinato, S. (eds) Opioid Receptors. Methods in Molecular Biology, vol 1230. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1708-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-1708-2_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1707-5

  • Online ISBN: 978-1-4939-1708-2

  • eBook Packages: Springer Protocols

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