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Agrobacterium Protocols pp 349–361Cite as

Tomato (Solanum lycopersicum)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Agrobacterium-mediated transformation is the most common method for the incorporation of foreign genes into the genome of tomato as well as many other species in the Solanaceae family. This chapter describes a protocol for the genetic transformation of tomato cultivar Micro-Tom using cotyledons as explants. Detailed procedures are also included for determining gene-copy number using a duplex qPCR TaqMan assay, and the histochemical analysis of GUS expression.

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Author information

Authors and Affiliations

  1. Plant Transformation Research Center, University of California, Riverside, CA, 92507-0124, USA

    Dora Garcia, Javier Narváez-Vásquez & Martha L. Orozco-Cárdenas Ph.D.

Authors
  1. Dora Garcia
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  2. Javier Narváez-Vásquez
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  3. Martha L. Orozco-Cárdenas Ph.D.
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Corresponding author

Correspondence to Martha L. Orozco-Cárdenas Ph.D. .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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Garcia, D., Narváez-Vásquez, J., Orozco-Cárdenas, M.L. (2015). Tomato (Solanum lycopersicum). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_28

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_28

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

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