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Agrobacterium Protocols pp 311–320Cite as

Pepper, Chili (Capsicum annuum)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1223))

Abstract

Pepper is a recalcitrant plant for Agrobacterium-mediated genetic transformation. Several obstacles to genetic transformation remain such as extremely low transformation rates; the choice of correct genotype is critical; and there is a high frequency of false positives due to direct shoot formation. Here, we report a useful protocol with a suitable selection method. The most important aspect of the pepper transformation protocol is selecting shoots growing from the callus, which is referred to as callus-mediated shoot formation. This protocol is a reproducible and reliable system for pepper transformation.

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Acknowledgment

This study was supported by a grant from the Next-Generation BioGreen21 research fund of the Rural Development of Administration from the Republic of Korea (PJ008178012014).

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Authors and Affiliations

  1. Biotechnology Institute, 113-141, Yanghwaro, Ganam, Yeoju, Gyeonggi, 469-885, South Korea

    Jung Min, Sun Hee Shin, En Mi Jeon, Jung Mi Park, Ji Young Hyun & Chee Hark Harn

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  1. Jung Min
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  2. Sun Hee Shin
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  3. En Mi Jeon
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  4. Jung Mi Park
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  5. Ji Young Hyun
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  6. Chee Hark Harn
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Corresponding author

Correspondence to Chee Hark Harn .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, Iowa, USA

    Kan Wang

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Min, J., Shin, S.H., Jeon, E.M., Park, J.M., Hyun, J.Y., Harn, C.H. (2015). Pepper, Chili (Capsicum annuum). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_25

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  • DOI: https://doi.org/10.1007/978-1-4939-1695-5_25

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1694-8

  • Online ISBN: 978-1-4939-1695-5

  • eBook Packages: Springer Protocols

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