Abstract
In most protocols for the Agrobacterium-mediated transformation of wheat, the preferred target tissues are immature embryos. However, transformation methods relying on immature embryos require the growth of plants under controlled conditions to provide a continuous supply of good-quality target tissue. The use of mature embryos as a target tissue has the advantage of only requiring good-quality seed as the starting material. Here we describe a transformation method based on the Agrobacterium-mediated transformation of callus cultures derived from mature wheat embryos of the genotype Bobwhite S56.
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Medvecká, E., Harwood, W.A. (2015). Wheat (Triticum aestivum L.) Transformation Using Mature Embryos. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1223. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1695-5_16
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DOI: https://doi.org/10.1007/978-1-4939-1695-5_16
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Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-1694-8
Online ISBN: 978-1-4939-1695-5
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