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Poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

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Abstract

Genetic engineering is an important tool for introducing desired genes into poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch). We describe in this chapter an Agrobacterium tumefaciens-mediated transformation protocol for poinsettia. A detailed description of genetic transformation, antibiotic selection, subsequent regeneration via somatic embryogenesis, and rooting as well as molecular and morphological analyses is included. The methodology described here could facilitate the future engineering of poinsettia for research purpose as well as commercial production of poinsettia plants with improved resistance or novel traits.

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Acknowledgments

Thanks are due to Sissel Haugslien, Dag-Ragnar Blystad, Erling Fløistad, and Carl Spetz for their practical support; CSIRO Plant Industry for providing pHANNIBAL and pART27 vectors; and J. Kristiansen nursery for the poinsettia plants. We are grateful to Dr. Nicholas Clarke for the linguistic correction. This research was supported by the Research Council of Norway grants 147147/140 and 199398 to Dr. Jihong Liu Clarke.

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Authors and Affiliations

  1. Bioforsk-Norwegian Institute for Agricultural and Environmental Research, Hogskoleveien 7, Ås, 1432, Norway

    M. Ashraful Islam, Tage Thorstensen & Jihong Liu Clarke

  2. Department of Horticulture, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    M. Ashraful Islam

Authors
  1. M. Ashraful Islam
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  2. Tage Thorstensen
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  3. Jihong Liu Clarke
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Corresponding author

Correspondence to Jihong Liu Clarke .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Islam, M.A., Thorstensen, T., Clarke, J.L. (2015). Poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_27

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_27

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

  • eBook Packages: Springer Protocols

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