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Cotton (Gossypium hirsutum L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

Abstract

Cotton continues to be a crop of great economic importance in many developing and some developed countries. Cotton plants expressing the Bt gene to deter some of the major pests have been enthusiastically and widely accepted by the farmers in three of the major producing countries, i.e., China, India, and the USA. Considering the constraints related to its production and the wide variety of products derived from the cotton plant, it offers several target traits that can be improved through genetic engineering. Thus, there is a great need to accelerate the application of biotechnological tools for cotton improvement. This requires a simple, yet robust gene delivery/transformant recovery system. Recently, a protocol, involving large-scale, mechanical isolation of embryonic axes from germinating cottonseeds followed by direct transformation of the meristematic cells has been developed by an industrial laboratory. However, complexity of the mechanical device and the patent restrictions are likely to keep this method out of reach of most academic laboratories. In this chapter, we describe the method developed in our laboratory that has undergone further refinements and involves Agrobacterium-mediated transformation of cotton cells, selection of stable transgenic callus lines, and recovery of plants via somatic embryogenesis.

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Acknowledgments

The authors wish to thank Ms. Lauren Tollack for excellent technical assistance. Experimental work in our laboratory has been supported by Cotton Inc., Texas Cotton Biotechnology Initiative (TxCOT), and Texas AgriLife Research.

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Authors and Affiliations

  1. Institute for Plant Genomics & Biotechnology, Texas A&M University, 2123 TAMU, College Station, TX, 77843-2123, USA

    Keerti S. Rathore, LeAnne M. Campbell & Shanna Sherwood

  2. Department of Soil & Crop Sciences, Texas A&M University, College Station, TX, 77843-2474, USA

    Keerti S. Rathore

  3. Faculty of Science of The University of Porto, Rua do Campo Alegre, s/n, 4169-007, Porto, Portugal

    Eugenia Nunes

Authors
  1. Keerti S. Rathore
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  2. LeAnne M. Campbell
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  3. Shanna Sherwood
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  4. Eugenia Nunes
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Corresponding author

Correspondence to Keerti S. Rathore .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Rathore, K.S., Campbell, L.M., Sherwood, S., Nunes, E. (2015). Cotton (Gossypium hirsutum L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_2

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

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