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Brassica rapa

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

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Abstract

Within this chapter we outline an A. tumefaciens-mediated transformation method for B. rapa using 4-day-old cotyledonary explants and the genotype R-o-18. Transformation efficiencies are typically achieved in the region of 1 % (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection.

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Acknowledgments

The authors acknowledge the support of the Biotechnology and Biological Science Research Council (BBSRC) Strategic Tools and Resources Grant BB/I023763/1 “Development of an efficient B. rapa transformation system to facilitate studies on fruit development in a diploid Brassica oilseed crop” and further support by BBSRC Strategic Programme Grant B/J004588/1 (GRO) and the John Innes Foundation.

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Authors and Affiliations

  1. Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK

    Tom Lawrenson, Cassandra Goldsack, Lars Ostergaard & Penny A. C. Hundleby née Sparrow

Authors
  1. Tom Lawrenson
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  2. Cassandra Goldsack
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  3. Lars Ostergaard
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  4. Penny A. C. Hundleby née Sparrow
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Corresponding author

Correspondence to Penny A. C. Hundleby née Sparrow .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Lawrenson, T., Goldsack, C., Ostergaard, L., Sparrow, P.A.C.H.n. (2015). Brassica rapa . In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_1

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

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