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Embryonic Stem Cell–Somatic Cell Fusion and Postfusion Enucleation

  • Huseyin Sumer
  • Paul J. Verma
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1222)

Abstract

Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fusion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.

Key words

Cell fusion Reprogramming Heterokaryons Postfusion enucleation 

Notes

Acknowledgements

We wish to acknowledge funding support from Dairy Australia, funding from the Dairy Futures CRC, and MIMR infrastructure funding supported by the Victorian Government’s Operational Infrastructure Support Program.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Monash Institute of Medical ResearchMonash UniversityClaytonAustralia
  2. 2.Turretfield Research CentreSouth Australian Research Institute (SARDI)RosedaleAustralia
  3. 3.Faculty of Science, Engineering and TechnologySwinburne University of TechnologyHawthornAustralia

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