Embryonic Stem Cell–Somatic Cell Fusion and Postfusion Enucleation
Embryonic stem (ES) cells are able to reprogram somatic cells following cell fusion. The resulting cell hybrids have been shown to have similar properties to pluripotent cells. It has also been shown that transcriptional changes can occur in a heterokaryon, without nuclear hybridization. However it is unclear whether these changes can be sustained following removal of the dominant ES nucleus. In this chapter, methods are described for the cell fusion of mouse tetraploid ES cells with somatic cells and enrichment of the resulting heterokaryons. We next describe the conditions for the differential removal of the ES cell nucleus, allowing for the recovery of somatic cells.
Key wordsCell fusion Reprogramming Heterokaryons Postfusion enucleation
We wish to acknowledge funding support from Dairy Australia, funding from the Dairy Futures CRC, and MIMR infrastructure funding supported by the Victorian Government’s Operational Infrastructure Support Program.