Abstract
Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Those experiments were designed to multiply high merit animals (Willadsen, Nature 320(6057):63–65, 1986; Prather et al., Biol Reprod 37(4):859–866, 1987; Wilmut et al., Nature 385(6619):810–813, 1997). Since then, cloning has provided us with a vast amount of knowledge and information on the reprogramming ability of somatic cells to different cell types which became an important basis for stem cell research and human medicine. Nowadays, the goals of most nuclear transfer work vary widely but in most cases the micromanipulation procedures remain the same. However, differences between species require different technical considerations. In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.
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References
Willadsen SM (1986) Nuclear transplantation in sheep embryos. (Translated from eng). Nature 320(6057):63–65
Prather RS et al (1987) Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. (Translated from eng). Biol Reprod 37(4):859–866
Campbell KH, Loi P, Otaegui PJ, Wilmut I (1996) Cell cycle co-ordination in embryo cloning by nuclear transfer. (Translated from eng). Rev Reprod 1(1):40–46
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Acknowledgements
The authors dedicate this book chapter to their late Ph.D. Supervisor, Professor Keith HS Campbell (1954–2012).
This work was partly carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009418022014 and PJ00911702014)” Rural Development Administration, Republic of Korea.
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Dedicated to Professor Keith HS Campbell
“Sheep in Scotland are very, very, very cheap.”
Keith HS Campbell, NY Times, 1997.
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Lee, JH., Maalouf, W.E. (2015). Nuclear Transfer in Ruminants. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_3
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DOI: https://doi.org/10.1007/978-1-4939-1594-1_3
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