Abstract
Pluripotent stem cells can be evaluated by pluripotent markers expression, embryoid body aggregation, teratoma formation, chimera contribution and even more, tetraploid complementation. Whether iPS cells in general are functionally equivalent to normal ESCs is difficult to establish. Here, we present the detailed procedure for chimera formation and tetraploid complementation, the most stringent criterion, to assessing pluripotency.
Xin Li, Bao-long Xia, and Wei Li contributed equally to this work.
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References
Tarkowski AK (1961) Mouse chimaeras developed from fused eggs. Nature 190:857–860
Gardner RL (1968) Mouse chimeras obtained by the injection of cells into the blastocyst. Nature 220:596–597
Buehr M, Meek S, Blair K et al (2008) Capture of authentic embryonic stem cells from rat blastocysts. Cell 135:1287–1298
Zhao XY, Li W, Lv Z et al (2009) iPS cells produce viable mice through tetraploid complementation. Nature 461:86–90
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Li, X., Xia, Bl., Li, W., Zhou, Q. (2015). Assessing Reprogramming by Chimera Formation and Tetraploid Complementation. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_18
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DOI: https://doi.org/10.1007/978-1-4939-1594-1_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1594-1
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