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Nuclear Reprogramming pp 227–245Cite as

Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1222))

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful method for mapping protein–DNA interactions in vivo. Genomic localization of histone modifications, transcription factors, and other regulatory proteins can be revealed by ChIP. However, conventional ChIP protocols require the use of large numbers of cells, which prevents the application of ChIP to rare cell types. We have developed ChIP assays suited for the immunoprecipitation of histone proteins or transcription factors from small cell numbers. Here we describe a rapid, yet sensitive micro (μ)ChIP protocol producing high signal to noise ratio output, suitable for as few as 100 cells. This chapter provides a detailed protocol for μChIP from early mammalian embryos, also suitable for any sample of limited numbers of cells. Minor modifications of this optimized high signal to noise ChIP protocol make it a reliable tool for the use with any cell number (100–107).

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Acknowledgements

Our work is supported by the Norwegian Cancer Society. We are thankful to Dr. Adam Robertson for reading and commenting on important parts of this chapter.

Author information

Authors and Affiliations

  1. Institute of Clinical Medicine, Division of diagnostics and intervention, Department of Microbiology, Oslo university hospital, Rikshospitalet, NO-0027, Oslo, Norway

    John Arne Dahl

  2. Clinic for Diagnostics and Intervention and Institute of Medical Microbiology, BIG CAS-OSLO Genome Research Cooperation, Oslo University Hospital, Rikshospitalet, Oslo, Norway

    Arne Klungland

Authors
  1. John Arne Dahl
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  2. Arne Klungland
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Corresponding author

Correspondence to John Arne Dahl .

Editor information

Editors and Affiliations

  1. INRA, Jouy-en-Josas, France

    Nathalie Beaujean

  2. INRA, Jouy-en-Josas, France

    Hélène Jammes

  3. INRA, Jouy-en-Josas, France

    Alice Jouneau

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Dahl, J.A., Klungland, A. (2015). Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1594-1_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1593-4

  • Online ISBN: 978-1-4939-1594-1

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