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Infectivity Assays of Human Rhinovirus-A and -B Serotypes

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Rhinoviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1221))

Abstract

Infectivity is a fundamental property of viral pathogens such as human rhinoviruses (HRVs). This chapter describes two methods for measuring the infectivity of HRV-A and -B serotypes: end point dilution (TCID50) assay and plaque assay. End point dilution assay is a quantal, not quantitative, assay that determines the dilution of the sample at which 50 % of the aliquots have infectious virus. It can be used for all the HRV-A and -B serotypes and related clinical isolates that grow in cell culture and induce cytopathic effect (CPE), degenerative changes in cells that are visible under a microscope. Plaque assay is a quantitative assay that determines the number of infectious units of a virus in a sample. After an infectious unit of virus infects one cell, the infected cell produces progeny viruses that then infect and kill a circle of adjacent cells. This circle of dead cells detaches from the dish and thus leaves a clear hole in a cell monolayer. Plaque assay works only for HeLa-adapted HRV-A and -B serotypes that can make visible plaques on the cell monolayer. Currently the end point dilution assay and plaque assay have not been developed for the newly discovered HRV-C.

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Authors and Affiliations

  1. Biological Mimetics Inc., 124 Byte Drive, Frederick, MD, 21702, USA

    Wai-Ming Lee

  2. Department of Pharmacology and Toxicology, School of Pharmacy, University of Arizona, Tucson, AZ, USA

    Yin Chen

  3. Global Biological Development, Bayer Healthcare, Berkeley, CA, USA

    Wensheng Wang

  4. Department of Medicine, University of Wisconsin, Madison, WI, USA

    Anne Mosser

Authors
  1. Wai-Ming Lee
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  2. Yin Chen
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  3. Wensheng Wang
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  4. Anne Mosser
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Corresponding author

Correspondence to Wai-Ming Lee .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Health, Nursing and Medical Sciences,, Monash University, Melbourne, Victoria, Australia

    David A. Jans

  2. Centre for Research in Therapeutic Solutions, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, Aust Capital Terr, Australia

    Reena Ghildyal

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Lee, WM., Chen, Y., Wang, W., Mosser, A. (2015). Infectivity Assays of Human Rhinovirus-A and -B Serotypes. In: Jans, D., Ghildyal, R. (eds) Rhinoviruses. Methods in Molecular Biology, vol 1221. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1571-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1571-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1570-5

  • Online ISBN: 978-1-4939-1571-2

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