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Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface

  • Shamaila AshrafEmail author
  • Rebecca Brockman-Schneider
  • James E. Gern
Protocol
  • 1.9k Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 1221)

Abstract

Rhinovirus-C (RV-C) were discovered recently using molecular methods. Classical methods failed to detect them since they could not grow in standard cell culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells of human airway at air-liquid interface (ALI). These cultures supported efficient replication of RV-C strains as determined by quantitative RT-PCR. This system has enabled study of the biological characteristics of RV-C strains, including quantitative research.

Key words

Rhinovirus Air-liquid interface Differentiated cultures Human airway epithelium Quantitative RT-PCR 

Notes

Acknowledgements

Funding for development of the ALI tissue culture system for RV-C was provided by NIH grant U19 AI070503.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Shamaila Ashraf
    • 1
    • 2
    Email author
  • Rebecca Brockman-Schneider
    • 3
  • James E. Gern
    • 4
  1. 1.Department of Pediatrics, School of Medicine and Public HealthUniversity of WisconsinMadisonUSA
  2. 2.American Type Culture Collection (ATCC)ManassasUSA
  3. 3.Department of Medicine, School of Medicine and Public HealthUniversity of WisconsinMadisonUSA
  4. 4.Department of Pediatrics and MedicineUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA

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