Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface

  • Shamaila AshrafEmail author
  • Rebecca Brockman-Schneider
  • James E. Gern
Part of the Methods in Molecular Biology book series (MIMB, volume 1221)


Rhinovirus-C (RV-C) were discovered recently using molecular methods. Classical methods failed to detect them since they could not grow in standard cell culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells of human airway at air-liquid interface (ALI). These cultures supported efficient replication of RV-C strains as determined by quantitative RT-PCR. This system has enabled study of the biological characteristics of RV-C strains, including quantitative research.

Key words

Rhinovirus Air-liquid interface Differentiated cultures Human airway epithelium Quantitative RT-PCR 



Funding for development of the ALI tissue culture system for RV-C was provided by NIH grant U19 AI070503.


  1. 1.
    Arden KE, Faux CE, O’Neill NT et al (2010) Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003. J Clin Virol 47:219–223PubMedCrossRefGoogle Scholar
  2. 2.
    Nicholson KG, Kent J, Ireland DC (1993) Respiratory viruses and exacerbations of asthma in adults. Br Med J 307:982–986CrossRefGoogle Scholar
  3. 3.
    Bizzintino J, Lee WM, Laing IA et al (2011) Association between human rhinovirus C and severity of acute asthma in children. Eur Respir J 37:1037–1042PubMedCrossRefPubMedCentralGoogle Scholar
  4. 4.
    Lee WM, Lemanske RF, Evans MD et al (2012) Human rhinovirus species and season of infection determine illness severity. Am J Respir Crit Care Med 186:886–891PubMedCrossRefPubMedCentralGoogle Scholar
  5. 5.
    Bochkov YA, Palmenberg AC, Lee WM et al (2011) Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C. Nat Med 17:627–632PubMedCrossRefPubMedCentralGoogle Scholar
  6. 6.
    Ashraf S, Brockman-Schneider R, Bochkov YA et al (2013) Biological characteristics and propagation of human rhinovirus-C in differentiated sinus epithelial cells. Virology 436:143–149PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Shamaila Ashraf
    • 1
    • 2
    Email author
  • Rebecca Brockman-Schneider
    • 3
  • James E. Gern
    • 4
  1. 1.Department of Pediatrics, School of Medicine and Public HealthUniversity of WisconsinMadisonUSA
  2. 2.American Type Culture Collection (ATCC)ManassasUSA
  3. 3.Department of Medicine, School of Medicine and Public HealthUniversity of WisconsinMadisonUSA
  4. 4.Department of Pediatrics and MedicineUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA

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