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Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions

  • Wai-Ming Lee
  • Yin Chen
  • Wensheng Wang
  • Anne Mosser
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1221)

Abstract

HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.

Key words

HeLa cells Suspension culture High-titer virus stock Purified virions 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Wai-Ming Lee
    • 1
  • Yin Chen
    • 2
  • Wensheng Wang
    • 3
  • Anne Mosser
    • 4
  1. 1.Biological Mimetics Inc.FrederickUSA
  2. 2.Department of Pharmacology and Toxicology, School of PharmacyUniversity of ArizonaTucsonUSA
  3. 3.Global Biological DevelopmentBayer HealthcareBerkeleyUSA
  4. 4.Department of MedicineUniversity of WisconsinMadisonUSA

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