Rhinoviruses pp 181-188 | Cite as

Mouse Models of Rhinovirus Infection and Airways Disease

  • Nathan W. Bartlett
  • Aran Singanayagam
  • Sebastian L. Johnston
Part of the Methods in Molecular Biology book series (MIMB, volume 1221)


Mouse models are invaluable tools for gaining insight into host immunity during virus infection. Until recently, no practical mouse model for rhinovirus infection was available. Development of infection models was complicated by the existence of distinct groups of viruses that utilize different host cell surface proteins for binding and entry. Here, we describe mouse infection models, including virus purification and measurement of host immune responses, for representative viruses from two of these groups: (1) infection of unmodified Balb/c mice with minor group rhinovirus serotype 1B (RV-1B) and (2) infection of transgenic Balb/c mice with major group rhinovirus serotype 16 (RV-16).

Key words

Purification Major group Minor group Serotype Hela cells In vivo infection 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Nathan W. Bartlett
    • 1
  • Aran Singanayagam
    • 1
  • Sebastian L. Johnston
    • 1
  1. 1.Airway Disease Infection Section, National Heart and Lung InstituteImperial College LondonLondonUK

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