Mast Cells pp 347-363 | Cite as

Real-Time Imaging of Ca2+ Mobilization and Degranulation in Mast Cells

Part of the Methods in Molecular Biology book series (MIMB, volume 1220)


Mast cells play a key role in allergy and inflammation processes as part of the immune response. The activation of mast cells via antigen binding and cross-linking of IgE receptors initiates the onset of dramatic calcium (Ca2+) mobilization dynamics that promote the release of mediators of inflammation and allergy. Ca2+ signaling in mast cells has been studied extensively using a variety of research tools and techniques. In these studies, a large number of proteins have been identified to participate in various stages of these processes.

Here we describe single-cell imaging as an important approach for examining Ca2+ signaling and exocytosis in mast cells. Single-cell imaging tools have advanced significantly over the last 10 years, in part due to improvements in microscope technology and in part due to the development of a new generation of Ca2+ indicators and genetically encoded Ca2+ sensors. The single-cell imaging techniques described here provide the spatial and temporal resolution required to decipher the signaling events that are critical for mast cell functions.

Key words

Live-cell imaging Calcium (Ca2+) signaling Calcium dynamics Mast cell degranulation 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Roy Cohen
    • 1
  • David A. Holowka
    • 2
  • Barbara A. Baird
    • 2
  1. 1.Baker Institute for Animal HealthCornell University College of Veterinary MedicineIthacaUSA
  2. 2.Department of Chemistry and Chemical BiologyCornell UniversityIthacaUSA

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