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Mast Cells pp 339–345Cite as

Measurement of Nitric Oxide in Mast Cells with the Fluorescent Indicator DAF-FM Diacetate

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1220))

Abstract

The production of nitric oxide in mast cells has been difficult to measure due to the low amounts made by mast cells, as well as limitations in the specificity and sensitivity of the assays available. We present here a sensitive and specific 96-well plate-based method to directly measure NO using the cell-permeable fluorescent compound DAF-FM diacetate.

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Author information

Authors and Affiliations

  1. Pulmonary Research Group, Department of Medicine, University of Alberta, 116th Street & 87 Avenue, Edmonton, AB, Canada, T6G 2R3

    Chris D. St. Laurent, Tae Chul Moon & A. Dean Befus

Authors
  1. Chris D. St. Laurent
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  2. Tae Chul Moon
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  3. A. Dean Befus
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Corresponding author

Correspondence to A. Dean Befus .

Editor information

Editors and Affiliations

  1. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Michael R. Hughes

  2. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Kelly M. McNagny

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© 2015 Springer Science+Business Media New York

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Laurent, C.D.S., Moon, T.C., Befus, A.D. (2015). Measurement of Nitric Oxide in Mast Cells with the Fluorescent Indicator DAF-FM Diacetate. In: Hughes, M., McNagny, K. (eds) Mast Cells. Methods in Molecular Biology, vol 1220. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1568-2_21

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  • DOI: https://doi.org/10.1007/978-1-4939-1568-2_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1567-5

  • Online ISBN: 978-1-4939-1568-2

  • eBook Packages: Springer Protocols

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