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Engineering Therapeutic Cancer Vaccines That Activate Antitumor Immunity

  • Per Ole Iversen
  • Mouldy Sioud
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1218)

Abstract

Vaccination represents one the most effective methods of preventing disease. Because dendritic cells (DCs) are the most efficient antigen presenting cells, exploiting their plasticity is likely to yield improved therapeutic vaccines. Herein, we applied a novel DC-based vaccine (i.e., DC loaded with leukemia antigens that have been transfected with an IL-10 siRNA capable of coordinately activating DCs via TLR7/8) in a rat model of acute myeloid leukemia. Leukemic rats treated with this new vaccine had less leukemic cell mass in their bone marrows and less extramedullar dissemination of the leukemic disease examined postmortem compared with rats given the control vaccine. Collectively, the new strategy demonstrates the possible usefulness of dual siRNAs as an immunomodulatory drug with antileukemic properties.

Key words

Dendritic cells siRNA Leukemia Immunotherapy 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of NutritionOslo University Hospital, NorwayMontebelloNorway
  2. 2.Department of ImmunologyOslo University Hospital, NorwayMontebelloNorway
  3. 3.Department of Immunology, Institute for Cancer ResearchThe Norwegian Radium HospitalMontebelloNorway

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