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MicroRNA In Situ Hybridization in Tissue Microarrays

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Book cover In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1211))

Abstract

In situ hybridization is used to visualize nucleic acids in microscopic tissue sections and has in recent years been used successfully to detect microRNAs. We have further optimized a technique to detect and semiquantitatively assay microRNA expression in tissue microarrays derived from formalin-fixed paraffin-embedded archival tumor tissue.

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References

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Correspondence to John P. C. Le Quesne .

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© 2014 Springer Science+Business Media New York

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Turnock-Jones, J.J., Le Quesne, J.P.C. (2014). MicroRNA In Situ Hybridization in Tissue Microarrays. In: Nielsen, B. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology, vol 1211. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1459-3_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1459-3_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1458-6

  • Online ISBN: 978-1-4939-1459-3

  • eBook Packages: Springer Protocols

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