Abstract
Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.
Key words
- In situ hybridization
- ISH
- GPCR
- NPY receptor/s
- Basal lingual epithelia
- Taste receptor cells
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References
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Hurtado MD, Acosta A et al (2012) Distribution of Y-receptors in murine lingual epithelia. PLoS One 7:e46358
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Zolotukhin, S. (2014). Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia. In: Nielsen, B. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology, vol 1211. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1459-3_17
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DOI: https://doi.org/10.1007/978-1-4939-1459-3_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1458-6
Online ISBN: 978-1-4939-1459-3
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