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Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia

Part of the Methods in Molecular Biology book series (MIMB,volume 1211)

Abstract

Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.

Key words

  • In situ hybridization
  • ISH
  • GPCR
  • NPY receptor/s
  • Basal lingual epithelia
  • Taste receptor cells

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  • DOI: 10.1007/978-1-4939-1459-3_17
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References

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  3. Hurtado MD, Acosta A et al (2012) Distribution of Y-receptors in murine lingual epithelia. PLoS One 7:e46358

    PubMed  CrossRef  CAS  PubMed Central  Google Scholar 

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Correspondence to Sergei Zolotukhin Ph.D. .

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Zolotukhin, S. (2014). Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia. In: Nielsen, B. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology, vol 1211. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1459-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1459-3_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1458-6

  • Online ISBN: 978-1-4939-1459-3

  • eBook Packages: Springer Protocols