Animal Models for Stem Cell Therapy pp 193-207 | Cite as
Bone Defect Repair in Mice by Mesenchymal Stem Cells
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Abstract
Adult bone marrow niche contains rare primitive but highly functional multipotent progenitors (e.g., mesenchymal stem cells; MSCs) capable of differentiating into specific mesenchymal tissues like bone, cartilage, muscle, fat tissues, ligament, dermis, bone marrow stroma, tendon, and other connective tissues. Upon in vivo transplantation, MSCs also secrete a wide range of growth factors, immunomodulatory cytokines, and important bioactive macromolecules including cell-derived exosomes to structure regenerative microenvironments. This protocol describes a mouse model to study bone formation/regeneration from adult mesenchymal stem cells.
Key words
Bone marrow Mesenchymal stem cells MSCs Bone defect Bone repair Bone formation Bone fracture regenerationNotes
Acknowledgments
We are grateful to Department of Biotechnology (DBT), Government of India for Ramalingaswami Fellowship and research support grant (DBT Grant #BT/PR 15420/MED/31/122/2011) to SK.
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