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A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA

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Book cover Advanced Protocols in Oxidative Stress III

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1208))

Abstract

Malondialdehyde (MA) is known to form from various lipids upon oxidation as one of secondary oxidation products. Determination of MA formed from lipid peroxidation has been used to examine occurrence of oxidative damages associated with many diseases, such as cancer, Alzheimer’s, arthritis, inflammation, diabetes, atherosclerosis, and AIDS as well as aging. Analysis of MA is, however, extremely difficult because it is highly reactive and readily polymerized and forming adducts with biological substances such as proteins, phospholipids, and DNA (Shibamoto, J Pharm Biomed Anal 41:12–25, 2002). Gas chromatographic method using stable derivative, 1-methylpyrazole was advanced and has been successfully used to analyze MA in various lipids and lipid-rich foods. This method was also applied to determine MA formed from DNA and related compounds. The amounts found in oxidized 2′-deoxyribonucleotides were 213.8 nmol/16 mmol in 2′-deoxyguanosine, 130.6 nmol/16 mmol in 2′-deoxycytidine, 85.1 nmol/16 mmol in 2′-deoxyadenosine, and 84.5 nmol/16 mmol in thymidine. When the antioxidant activity of flavonoids and anthocyanins against calf thymus DNA oxidized with Fenton’s reagent was examined using this newly developed gas chromatographic method, antioxidant activity of flavonoids and anthocyanins ranged from 48.5 % (catechin) to 29.9 % (apigenin) and from 45.0 % (callistephin) to 10.2 % (cyaniding), respectively.

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Authors and Affiliations

  1. Department of Environmental Toxicology, University of California, 4115 Meyer Hall, One Shields Avenue, Davis, CA, 95616, USA

    Takayuki Shibamoto

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  1. Takayuki Shibamoto
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Correspondence to Takayuki Shibamoto .

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Editors and Affiliations

  1. University of Florida, Gainsville, Florida, USA

    Donald Armstrong

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Shibamoto, T. (2015). A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress III. Methods in Molecular Biology, vol 1208. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1441-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1441-8_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1440-1

  • Online ISBN: 978-1-4939-1441-8

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