Abstract
The cytotoxic peroxynitrite (ONOO−) is an oxidation product of the cytoprotective nitric oxide (NO). Our studies support the hypothesis that the concentration ratio of NO and ONOO−, [NO]/[ONOO−] can be a marker of nitroxidative imbalance, which subsequently correlates well with endothelial dysfunction and dysfunction of the cardiovascular system. Nanosensors, described here, have been used for simultaneous monitoring and measurement of NO and ONOO− release from a single endothelial cell. These nanosensors, with a diameter of 200–300 nm, can be positioned accurately in close proximity of 5–10 μm from the endothelial cell membrane. The response time of the sensors is better than a millisecond and the detection limit is 10−9 M, with a linear concentration response of up to about 2 μM. The application of these sensors for the measurement of the balance and imbalance of [NO]/[ONOO−] in normal and dysfunctional endothelium is demonstrated.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Malinski T, Taha Z (1992) Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor. Nature 358:676–678
Malinski T, Radomski MW, Taha Z, Moncada S (1993) Direct electrochemical measurement of nitric oxide released from human platelets. Biochem Biophys Res Commun 194:960–965
Balligand JL, Ungureanu-Longrois D, Simmons WW, Pimental D, Malinski TA, Kapturczak M, Taha Z, Lowenstein CJ, Davidoff AJ, Kelly RA et al (1994) Cytokine-inducible nitric oxide synthase (iNOS) expression in cardiac myocytes. Characterization and regulation of iNOS expression and detection of iNOS activity in single cardiac myocytes in vitro. J Biol Chem 269:27580–27588
Kanai AJ, Strauss HC, Truskey GA, Crews AL, Grunfeld S, Malinski T (1995) Shear stress induces ATP-independent transient nitric oxide release from vascular endothelial cells, measured directly with a porphyrinic microsensor. Circ Res 77:284–293
Pinsky DJ, Patton S, Mesaros S, Brovkovych V, Kubaszewski E, Grunfeld S, Malinski T (1997) Mechanical transduction of nitric oxide synthesis in the beating heart. Circ Res 81:372–379
Kidd GA, Dobrucki LW, Brovkovych V, Bohr DF, Malinski T (2000) Nitric oxide deficiency contributes to large cerebral infarct size. Hypertension 35:1111–1118
Malinski T (2000) In situ detection of nitric oxide. Methods Mol Med 36:133–144
Malinski T, Taha Z, Grunfeld S, Patton S, Kapturczak M, Tomboulian P (1993) Diffusion of nitric oxide in the aorta wall monitored in situ by porphyrinic microsensors. Biochem Biophys Res Commun 193:1076–1082
Cosentino F, Patton S, D’Uscio LV, Werner ER, Werner-Felmayer G, Moreau P, Malinski T, Luscher TF (1998) Tetrahydrobiopterin alters superoxide and nitric oxide release in prehypertensive rats. J Clin Invest 101:1530–1537
Huk I, Nanobashvili J, Neumayer C, Punz A, Mueller M, Afkhampour K, Mittlboeck M, Losert U, Polterauer P, Roth E, Patton S, Malinski T (1997) L-arginine treatment alters the kinetics of nitric oxide and superoxide release and reduces ischemia/reperfusion injury in skeletal muscle. Circulation 96:667–675
Van Der Loo B, Labugger R, Skepper JN, Bachschmid M, Kilo J, Powell JM, Palacios-Callender M, Erusalimsky JD, Quaschning T, Malinski T, Gygi D, Ullrich V, Luscher TF (2000) Enhanced peroxynitrite formation is associated with vascular aging. J Exp Med 192:1731–1744
Soneja A, Drews M, Malinski T (2005) Role of nitric oxide, nitroxidative and oxidative stress in wound healing. Pharmacol Rep 57 Suppl:108–119
Malinski T (2007) Nitric oxide and nitroxidative stress in Alzheimer’s disease. J Alzheimers Dis 11:207–218
Pryor WA, Lemercier JN, Zhang H, Uppu RM, Squadrito GL (1997) The catalytic role of carbon dioxide in the decomposition of peroxynitrite. Free Radic Biol Med 23:331–338
Kiechle FL, Malinski T (1993) Nitric oxide. Biochemistry, pathophysiology, and detection. Am J Clin Pathol 100:567–575
Malinski T, Huk I (2001) Measurement of nitric oxide in single cells and tissue using a porphyrinic microsensor. Curr Protoc Neurosci Chapter 7:Unit7.14
Brovkovych V, Patton S, Brovkovych S, Kiechle F, Huk I, Malinski T (1997) In situ measurement of nitric oxide, superoxide and peroxynitrite during endotoxemia. J Physiol Pharmacol 48:633–644
Mason RP, Kalinowski L, Jacob RF, Jacoby AM, Malinski T (2005) Nebivolol reduces nitroxidative stress and restores nitric oxide bioavailability in endothelium of black Americans. Circulation 112:3795–3801
Kubant R, Malinski C, Burewicz A, Malinski T (2006) Peroxynitrite/nitric oxide balance in ischemia/reperfusion injury-nanomedical approach. Electroanalysis 18:410–416
Bennett JE, Wheeler DE, Czuchajowski L, Malinski T (1989) A novel meso-[2.2]paracyclophanyltriphenylporphyrin—electrochemical oxidation and conductive polymeric film formation. J Chem Soc Chem Comm:723–725
Burewicz A, Dawoud H, Jiang L-L, Malinski T (2013) Nitric oxide/peroxynitrite redox imbalance in endothelial cells measured with amperometric nanosensors. Am J Anal Chem 4:30–36
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Malinski, T. (2015). Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress III. Methods in Molecular Biology, vol 1208. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1441-8_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1441-8_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1440-1
Online ISBN: 978-1-4939-1441-8
eBook Packages: Springer Protocols