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Protein Microarrays for Parasite Antigen Discovery

  • Patrick Driguez
  • Denise L. Doolan
  • Douglas M. Molina
  • Alex Loukas
  • Angela Trieu
  • Phil L. Felgner
  • Donald P. McManusEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1201)

Abstract

The host serological profile to a parasitic infection, such as schistosomiasis, can be used to define potential vaccine and diagnostic targets. Determining the host antibody response using traditional approaches is hindered by the large number of putative antigens in any parasite proteome. Parasite protein microarrays offer the potential for a high-throughput host antibody screen to simplify this task. In order to construct the array, parasite proteins are selected from available genomic sequence and protein databases using bioinformatic tools. Selected open reading frames are PCR amplified, incorporated into a vector for cell-free protein expression, and printed robotically onto glass slides. The protein microarrays can be probed with antisera from infected/immune animals or humans and the antibody reactivity measured with fluorophore labeled antibodies on a confocal laser microarray scanner to identify potential targets for diagnosis or therapeutic or prophylactic intervention.

Key words

Schistosomiasis Protein microarray Parasite Antibody/serum screening Vaccine and diagnostic discovery 

Notes

Acknowledgements

We want to thank all the staff at Antigen Discovery Incorporated and the Protein Microarray Laboratory, University of California, Irvine. We also wish to thank Hamish McWilliam for his illustrations of schistosome lifecycle stages. PLF’s research was supported by National Institute of Allergy and Infectious Disease Grants U54AI065359 and U01AI078213. DLD, AL, and DPM receive support from the National Health and Medical Research Council of Australia.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Patrick Driguez
    • 1
  • Denise L. Doolan
    • 2
  • Douglas M. Molina
    • 3
  • Alex Loukas
    • 4
  • Angela Trieu
    • 2
  • Phil L. Felgner
    • 5
  • Donald P. McManus
    • 1
    Email author
  1. 1.Molecular Parasitology LaboratoryQIMR Berghofer Medical Research InstituteHerstonAustralia
  2. 2.Molecular Vaccinology LaboratoryQIMR Berghofer Medical Research InstituteHerstonAustralia
  3. 3.Antigen Discovery IncorporatedIrvineUSA
  4. 4.Centre for Biodiscovery and Molecular Development of TherapeuticsJames Cook UniversityCairnsAustralia
  5. 5.Department of MedicineUniversity of California IrvineIrvineUSA

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