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Techniques to Study Epigenetic Control and the Epigenome in Parasites

  • Sheila C. Nardelli
  • Li-Min Ting
  • Kami KimEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1201)

Abstract

Epigenetics is the study of heritable changes in gene expression that occur independent of the DNA sequence. Due to their intimacy with DNA, histones have a central role in chromatin structure and epigenetic regulation. Their tails are subject to posttranslational modifications (PTMs) that together with chromatin-remodeling proteins control the access of different proteins to DNA and allow a precise response to different environmental conditions. The first part of this chapter is dedicated to histone enrichment methods that allow the study of histones using techniques such as immunoblot or mass spectrometry for the mapping of the histone PTM network. Next we describe chromatin immunoprecipitation-based techniques (ChIP) for study of the epigenome. ChIP followed by microarray or next-generation sequencing enables the precise genomic localization of protein-DNA interactions. These techniques for genome-wide profiling of chromatin provide powerful and efficient tools to study the epigenome.

Key words

Histone Chromatin immunoprecipitation Microarray Sequencing 

Notes

Acknowledgement

This work was supported by NIH grants RC4AI092801 (KK), R01AI087625 (KK), and 5T32AI070117-04 (SCN). We thank members of the Kim laboratory for review and helpful suggestions for this chapter.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Instituto Carlos Chagas-FiocruzCuritiba - PRBrazil
  2. 2.Albert Einstein College of MedicineBronxUSA

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