Abstract
The neural crest cells give rise to neurons and glia in the peripheral nervous system, which is an important component of the nervous system. Here we developed a scalable process of inducing neural crest stem cells (NCSCs) from hESCs/iPSCs by a combination of growth factors in medium conditioned on stromal cells, and showed that NCSCs could be purified by p75 using FACS. In vitro-expanded NCSCs were able to differentiate into neurons and glia (Schwann cells) of the peripheral nervous system (PNS) as well as mesenchymal derivatives.
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Acknowledgements
This work was supported in part by the California Institute for Regenerative Medicine Grants CL1-00501-1 (Zeng) and TG2-01155 (Zeng) and Maryland Stem Cell Research Fund Grant#302385 (Hoke). We thank Drs. L. Cheng at the Johns Hopkins University for providing the iPSC line and J. Peng for technical assistance. We also acknowledge the Developmental Studies Hybridoma Bank maintained by the University of Iowa (Iowa City, IA) for supply of monoclonal antibodies.
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Liu, Q., Swistowski, A., Zeng, X. (2014). Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells. In: Kioussi, C. (eds) Stem Cells and Tissue Repair. Methods in Molecular Biology, vol 1210. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1435-7_7
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DOI: https://doi.org/10.1007/978-1-4939-1435-7_7
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