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Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for In Vivo Analysis of the Brain Tumor-Initiating Cell

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Stem Cells and Tissue Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1210))

Abstract

The generation of xenograft models, which support the growth of human tissue in animals, forms an important part of a researcher’s tool kit and enhances the ability to understand the initiation and development of cancer in vivo. Especially in the context of the brain tumor-initiating cell (BTIC), a xenograft model allows for careful characterization of BTIC roles in tumor initiation, growth, and relapse. Here, we detail a set of procedures which describe the isolation, enrichment, and intracranial injection of human BTICs from patient samples to generate xenograft models of a human brain tumor.

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Authors and Affiliations

  1. McMaster Children’s Hospital, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1

    Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora & Sheila K. Singh

Authors
  1. Maleeha Qazi
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  2. Aneet Mann
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  3. Randy van Ommeren
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  4. Chitra Venugopal
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  5. Nicole McFarlane
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  6. Parvez Vora
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  7. Sheila K. Singh
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Corresponding author

Correspondence to Sheila K. Singh .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, Oregon, USA

    Chrissa Kioussi

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© 2014 Springer Science+Business Media New York

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Qazi, M. et al. (2014). Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for In Vivo Analysis of the Brain Tumor-Initiating Cell. In: Kioussi, C. (eds) Stem Cells and Tissue Repair. Methods in Molecular Biology, vol 1210. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1435-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1435-7_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1434-0

  • Online ISBN: 978-1-4939-1435-7

  • eBook Packages: Springer Protocols

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